Investigation of the potential use of essential oils as pesticides using Daphnia magna as a model organism

  • Poster Presentation
  • Poster 2 (Flash Talk: 11 Jun 2018 14:41)
  • Foyer, UCD Agriculture and food science Centre
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Kyle Sadyk*
Cork Institute of Technology

Joseph Croke
Cork Institute of Technology

*Presenting Author

In this study, essential oils were investigated for their potential as possible leads to the development of safer more environmentally friendly pesticides. Essential oils are secondary metabolites produced by plants for many purposes including protection against other organisms. In this study, the possible pesticidal attributes of essential oils were investigated. Effective concentrations (EC50) of Rosemary (Rosmarinus officinalis), Grapefruit (Citrus paradise), Orange (Citrus sinensis) and Spearmint (Mentha spicate) essential oils were investigated. The aquatic toxicity of the oils was examined using Daphnia magna, an aquatic fresh water organism commonly used in environmental and ecological chemical toxicity investigation and as used by the Organisation for Economic Co-operation and Development (OECD). The Daphnia magna acute immobilization test (OECD 202) was used in this study. Daphnia magna were grown and treated with the various essential oils and visually assessed for immobilisation at 24 and 48 hours after exposure. Preliminary toxicity immobilisation test results showed Orange and Grapefruit essential oils showed the greatest activity followed by Rosemary and Spearmint oils. Further investigation using an Acetylcholinesterase bio-assay to attempt to identify a possible mechanism of action is being undertaken and is hoped to be presented.