On the mechanism of the cell cycle arrest and apoptosis induction of suspension-cultured tobacco cells after exposure to the static magnetic field

  • Poster Presentation
  • Poster 23 (Flash Talk: 11 Jun 2018 17:12)
  • Foyer, UCD Agriculture and food science Centre
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Farzaneh Mohammadi
Tarbiat Modares University

Faezeh Ghanati
Tarbiat Modares University

Mohsen Sharifi
Tarbiat Modares University

Najmeh Ahmadian Chashmi
Mazandaran University

Mohammad Pormehr*
Tarbiat Modares University
University College Dublin

Paul Mc Cabe
University College Dublin

*Presenting Author

One of the important effects of the magnetic field is the change of cell cycle progression, although the cell response intensity depends on the cell type and the duration of treatment. The effect of 0.2 mT SMF on cell cycle progression of synchronized suspension-cultured tobacco cells and the molecular mechanism involved in their response to SMF was studied. After exposing, the nuclei were released from the protoplast pellet in woody plant buffer (WPB), fixed, stained and their percent of cell cycle phases; G1, S, G2/M were elucidated using flow cytometer instrument. Exposure to SMF delayed G1/S transition accompanied by a decrease in the expression of cyclin-dependent kinases A and D-type cyclin, but an increase in the adenylyl cyclase (AC), transcription factor E2F, retinoblastoma protein (Rbp), and CDK-inhibitor protein 21 (p21) genes. Retardation of G1/S transition in the SMF-treated tobacco cells may be associated with an increase in the nitric oxide (NO), hydrogen peroxide (H2O2) radicals, salicylic acid (SA) and apoptosis contents. In this study, we will firstly support the role of SMF on cell cycle progression tobacco cells and secondly investigate the amount of internal H2O2, NO and SA in physiological and stress conditions in cells.

Keywords: Cell cycle progression; Magnetic field; Nicotiana tabacum; Nitric oxide; salicylic acid