Barley Against Rhynchospoirum Disease (BARD): a new project to identify novel sources of resistance to R. commune.

  • Poster Presentation
  • Poster 21 (Flash Talk: 11 Jun 2018 17:10)
  • Foyer, UCD Agriculture and food science Centre
  • View all IPSAM abstracts

Tara O'Connor*
Maynooth University
Teagasc Crops, Environment and Land Use Programme

Emmanuelle Graciet
Maynooth University

Ewen Mullins
Teagasc Crops, Environment and Land Use Programme

*Presenting Author

The activation of a plant’s immune system relies on the ability of its receptors to detect a range of pathogen molecules. Besides the importance of these receptors and the associated downstream cellular events, the importance of susceptibility genes (S-genes) has emerged in recent years. The product of S-genes are exploited by pathogens to promote infection. Crops deficient for specific S-genes are more resistant to pathogens. Thus, inactivating S-genes could deliver a more durable resistant phenotype and provide a novel strategy for crop defence over existing systems that rely solely on resistance genes. This project will identify and inactivate novel S-genes in barley to generate barley cultivars that are more resistant to the pathogen Rhynchosporium commune. Barley orthologs of known S-genes (across monocots and dicots) will be identified using sequence similarity searches with known S-genes. In parallel to this in silico approach, higher-confidence S-genes will be selected by generating and analysing transcriptomic datasets generated from studying compatible and incompatible barley – R. commune interactions. The function of these ‘high-confidence’ S-genes during R. commune infection will be assessed, and promising candidate S-genes will then be interrupted via editing. A full agronomic profile of the resulting lines will be generated, including their response to R. commune and other biotic/abiotic stresses.