Screening small secreted hypothetical proteins of Z. tritici using a non-host plant N. benthamiana.

  • Oral Presentation
  • Plant Pathology
  • 12 Jun 2018 09:20
  • FS-G01, UCD Agriculture and food science Centre
  • View all IPSAM abstracts

Sujit Jung, Karki*
School of Agriculture and Food Science, Earth Institute, University College Dublin

Angela Feechan
School of Agriculture and Food Science, Earth Institute, University College Dublin

*Presenting Author

Zymoseptoria tritici, an ascomycete fungus that causes Septoria Tritici Blotch, is a major threat to wheat production worldwide. Infection occurs through the stomata, followed by a prolonged latent period where Z. tritici remains within the plant apoplast. The fungus then shifts to a necrotrophic phase, resulting in necrosis of the plant tissue corresponding with an increase in fungal biomass. Z. tritici is predicted to produce a series of small secreted proteins (ZtSSP) throughout its interaction with the host (Rudd et al. 2015). In this study we mined publically available dataset from Amaral et al 2012 and using NCBI Blastp and conserved domain search, identified 52 ZtSSP that showed no homology to known proteins. 30 of these ZtSSPs were cloned into overexpression vectors for transient expression in planta. In order to test these SSPs as potential effectors, the induction of cell death in the non-host model plants Nicotiana benthamiana was tested. We identified 9 candidates that resulted in cell death phenotype. 4 of the candidates were previously reported as cell death inducers by Kettles et al 2017. Transient overexpression of these candidate resulted in rapid accumulation of H202 and up-regulation of defense-associated gene. The differences in the expression patterns of these candidate genes suggested they might play different role during colonisation of host.