SIPK's roles in inducing the antioxidant system till the program cell death in cadmium-treated tobacco cells

  • Poster Presentation
  • Poster 24 (Flash Talk: 11 Jun 2018 17:13)
  • Foyer, UCD Agriculture and food science Centre
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Mohammad Pormehr*
Tarbiat Modares University
University College Dublin

Faezeh Ghanati
Tarbiat Modares University

Mohsen Sharifi
Tarbiat Modares University

Farzaneh Mohammadi
Tarbiat Modares University

Paul Mc Cabe
University College Dublin

*Presenting Author

Cadmium (Cd) is a toxic metallic element that induces oxidative stress and program cell death. This study was designed to investigate the role of SIPK signalling pathway in the tobacco cells during of Cd stress. In this regard, the expression of genes salicylic acid-induced protein kinase (SIPK), Hsr 203J and catalase (CAT), along with physiological parameters including salicylic acid (SA), apoptosis, reactive oxygen species (ROS), catalase and caspase 3/7 activity in the presence of Cd and MAPK inhibitor, were investigated at 3, 6, 12 and 24 h after treatments. The results indicate that the SIPK signalling pathway induces the expression of the catalase gene and its enzyme activity (as an antioxidant enzyme) at 3 h after the Cd treatments, while in the presence of inhibitor, expression gene and enzyme activity are suppressed. Also, Hsr203J gene expression is highest at the peak of cadmium-induced apoptosis. But in Cd & inhibitor treatments, there is a time shift in the peak of apoptosis that is consistent with expression peak of the Hsr 203J gene. This time shift was observed in the amount of ROS and SA and caspase 3/7 activity. In the interpretation of the results, it could be suggested that salicylic acid, by interfering with the SIPK signalling pathway in the early hours of stress, induces an antioxidant system and, in the longer term, by inhibiting the antioxidant system, increases the ROS and HR gene expression.

Keywords: Salicylic acid-induced protein kinase; SIPK signalling pathway; salicylic acid; Cd-induced apoptosis; Nicotiana tabacum